Unanchored ubiquitin chains
WebUnanchored chains of ubiquitin have recently been proposed to play a role in signaling. In this issue of Immunity, Jiang et al. (2012) demonstrate that binding of free polyubiquitin … Web14 Apr 2024 · Spinocerebellar ataxia type 3 (SCA3), also known as Machado–Joseph disease, is the most common dominantly inherited ataxia. SCA3 is caused by a CAG repeat expansion in the ATXN3 gene that encodes an expanded tract of polyglutamine in the disease protein ataxin-3 (ATXN3). As a deubiquitinating enzyme, ATXN3 regulates …
Unanchored ubiquitin chains
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Web6 Jul 2015 · MJ Lee, BH Lee, J Hanna, RW King, D Finley, Trimming of ubiquitin chains by proteasome-associated deubiquitinating enzymes. Mol Cell Proteomics 10 ... R Hao, et al., … WebIsoleucine 44 Hydrophobic Patch Controls Toxicity of Unanchored, Linear Ubiquitin Chains through NF-κB Signaling. Journals. Active Journals Find a Journal Proceedings Series. ... our results showed the presence of ubiquitin-related proteins including 3 isoforms of ubiquitin-protein ligase (E3), ubiquitin-associated (UBA) domain-containing ...
WebNEDD8 is a ubiquitin-like protein that activates cullin-RING E3 ubiquitin ligases (CRLs). Here, we identify a novel role for NEDD8 in regulating the activity of poly(ADP-ribose) … WebPurification of Unanchored Polyubiquitin Chains from Influenza Virions Authors: Yasuyuki Miyake 1 , 2 , Patrick Matthias 3 , Yohei Yamauchi 2 Yasuyuki Miyake 1 , 2 ... The virion …
Web30 May 2024 · Ubiquitin chains can be attached onto other proteins or can exist as unanchored species, i.e. free from another protein. Unanchored ubiquitin chains are … Web7 Sep 2010 · DUBs vary in their preference for cleaving ubiquitin chains of different linkages types, their preference for substrate-bound ubiquitin chains as opposed to unanchored (free) ubiquitin chains, and their cellular localization . Some DUBs appear to function with high specificity toward one or a few substrates, although only a few examples of this are …
Web11 Jan 2012 · The method is based on the formation of unanchored Ub chains by E1 and a specific E2/E3 enzyme and separation of the products by cation exchange chromatography. ... The Ball and Chain of...
WebUbiquitination assays using Ark 68 aa and Ark 119 aa with the complex of the two E2 enzymes UBCH13-MMS2 revealed that the Ark 119 polypeptide enhances the formation of unanchored K63-linked poly-ubiquitin chains beyond those observed for RING or UBCH13/MMS2 alone . The above results indicate that non-RING elements are significant … isaidub money heistWebDepartment of Microbiology and Immunology RESEARCH ACTIVITIES: (2024-Present) Graduate student collaborator with Dr. Ricardo Rajsbaum PhD: NIH Grant: “Unanchored Polyubiquitin Chains and Innate ... olfactory epithelium and bulbWeb24 May 2024 · Isoleucine 44 Hydrophobic Patch Controls Toxicity of Unanchored, Linear Ubiquitin Chains through NF-κB Signaling Cells June 22, 2024 See publication. Expanding Role of Ubiquitin in Translational ... olfactory epithelium histology mouseWeb26 Oct 2024 · Unanchored ubiquitin chains may also associate with ubiquitin-binding proteins in a “reserve” pool for later use in any of the other suggested routes of … olfactory epithelium is an example ofWebUnanchored polyubiquitin chains are endogenous non-substrate linked ubiquitin polymers which have emerging roles in the control of cellular physiology. We describe an affinity purification method based on an isolated ubiquitin-binding domain, the olfactory factory couponWebHere, we present methods using purified ZnF-UBP domain of HDAC6 to immunoprecipitate viral unanchored ubiquitin chains, which can then be used for further biochemical … olfactory epithelium partsWebUnanchored ubiquitin chains packaged within the virion help direct the core to the cellular aggresome where mechanical forces have been proposed to accelerate uncoating and release of vRNPs into the cytosol (Banerjee et al., 2014) . Our data now implicate EPS8 as another host factor important during these later stages of uncoating. olfactory evoked potentials