Stat5 reporter assay
WebMoreover, bioinformatics analyses and luciferase reporter assay indicated that exosomal miR-182-5p was bound to PPARG 3' UTR sequence and inhibited the expression of PPARG. Collectively, our findings provided a new role of EPCs-EXO in the clinical treatment of diabetic skin wounds. ... (STAT5) to control EPC cell-cycle progression 44. In ... WebAug 15, 2003 · Stat5 deficiency reduces survival and proliferation of developing mast cells. (A) Wild-type and Stat5-deficient bone marrow cells were cultured for the indicated times …
Stat5 reporter assay
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WebMar 19, 2013 · Reporter assay to monitor oncogenic FLT3-ITD activity. As FLT3-ITD strongly activates STAT5, 3, 4, 12, 13, 20, 24, 26 we employed a FLT3-ITD-driven STAT5 activation reporter assay for the screen ... Webperforming dual-luciferase reporter assays. 3) Firefly Luciferase (Fluc) Lentivirus (BPS Bioscience, #79692-G, #79692-H, #79692-P): ... STAT5 Reporter (Luc)-BaF3 cell line 79772 2 vials ONE-Step™ Luciferase Assay System 60690-1 10 ml ONE-Step™ Luciferase Assay System 60690-2 100 ml ...
WebThis assay utilized the growth factor-dependent human erythroleukemia TF1 cell line engineered to express a green fluorescent protein-STAT5 fusion protein. Furthermore, a complementary beta-lactamase reporter gene assay was developed to analyze the transcriptional activity of STAT5 downstream of JAK2 in TF1 cells. WebMay 1, 2011 · One day after transfection, β-galactosidase reporter assays were performed. Specific activities from cells transfected with p ... Stat5 reporter gene expression in tissue sections from liver, kidney, spleen, thymus, and uterus. Indicated organs were isolated from C57BL/6 wild-type mice (row 1) and Stat5-REIN reporter mice (rows 2 and 3 ...
WebSTAT5 reporter system is designed to monitor the STAT5 transcriptional activity. STAT5 reporter assay services include screening of STAT5 agonists/antagonists, EC50/IC50 evaluation of those molecules, and functional analysis of STAT5 signaling pathways. Assay results will contain a written report and downloadable Excel- or Prism-based data. WebSTAT5 reporter assay services include screening of STAT5 agonists/antagonists, EC50/IC50 evaluation of those molecules, and functional analysis of STAT5 signaling …
WebAssay Entry Cell-based terbium phospho-specific activity (stable) Cell State Dividing Cells Detection Method Fluorescent Gene Symbol STAT5A Optimal Response GM-CSF (EC50 = …
WebOct 23, 2024 · Reporter assays demonstrated that Shd1 repressed Stat5-mediated transcription in a dose-dependent manner without affecting Stat5 stability or phosphorylation. Nakajima et al. (2009) concluded that SHD1 is a cytokine-inducible negative-feedback regulator of STAT5. da トラッキングWebSTAT Reporter Cell Line - Tool for Screening of Drugs Targeting JAK/STAT Pathway; Imaging with CRISPR/Cas9; CoV-S Pseudotyped Lentivirus - Efficient Tool for Anti-CoV … daとは 自動車WebIn an attempt to use the cells for large-scale chemical screens to identify JAK3 inhibitors, we established a cell line, 32D/IL-2Rβ/6xSTAT5, stably expressing a STAT5 reporter gene. Treatment of this cell line with IL-2 or IL-3 dramatically increased the reporter activity in a high-throughput format. da なんの略WebUpon IL-2 or IL-15 stimulation, levels of STAT5-induced SEAP can be readily monitored using QUANTI-Blue™ Solution. HEK-Blue™ IL-2 cells and HEK-Blue™ CD122/CD132 cells … da トリビュートWebFeb 2, 2024 · STAT5 is a key member of the JAK/STAT core cancer pathway, activated by a plethora of cytokines and growth factors to regulate a wide spectrum of physiologic … da ノクチル 編成WebDec 1, 1999 · A highly sensitive method for determination of insulin receptor (IR) kinase activity in whole cells, which is based on a STAT5 (signal transducer and activator of transcription 5)-dependent reporter gene assay, has been developed. We show in Rat1 fibroblasts stably overexpressing the human IR (Rat1- … da とは 車WebThe following assays are designed for 96-well format. To perform the assay in a different format, the cell number and reagent volume should be scaled appropriately. A. Dose response of STAT5 reporter (Luc) -U937 cells to human GM-CSF 1. Harvest STAT5 reporter (Luc)-U937 cells from culture in the Growth Medium 8A and seed cells at a da とは it