WebMar 15, 2024 · With the development of Az-C14-coenzyme A (CoA), combined with mass spectrometry, the Berthiaume group uncovered for the first time endogenously palmitoylated proteins that are enriched in rat liver mitochondrial matrix, including 19 novel proteins and two previously characterized liver mitochondrial matrix proteins … WebChapter 1 presents an introduction to quantitative LC-MS/MS-based protein analysis, including instrumentation and techniques relevant to the work presented in Chapters 2 …
emPAI Calc—for the estimation of protein abundance from large …
WebSummary: emPAI Calc is an open-source web application for the estimation of protein abundance. It uses the correlation between the number of identified peptides and protein abundance in mass spectrom WebPalmitoylation is typically detected by monitoring incorporation of radiolabeled palmitate (3 H-palmitate) or palmitate analogs (125 I-IC16 palmitate) into the protein of interest (14, … iowa falls ia restaurants
Sheetal Uppal - Research Fellow - National Eye Institute (NEI ...
WebDec 17, 2013 · Direct detection and quantification of protein/peptide palmitoylation by mass spectrometry (MS) is a challenging task because of the tendency of palmitoyl loss during sample preparation and tandem MS analysis. In addition, the large difference in … WebClick-chemistry-based chemical probes for the detection of protein palmitoylation hasten the discovery of novel palmitoylated proteins and elucidate their biological functions. 21 The combination of mass-spectrometry-based quantitative proteomics with click chemistry probes can be used to explore the dynamics of protein palmitoylation under different … WebPalmitoylation is a process by which proteins are modified post-translationally by the covalent attachment of saturated 16-carbon (palmitoyl) ... mass spectrometry (FABS) … iowa falls ia county